1WB7
Iron Superoxide Dismutase (Fe-SOD) From The Hyperthermophile Sulfolobus Solfataricus. Crystal Structure of the Y41F mutant.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-10-24 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 75.188, 121.753, 59.420 |
Unit cell angles | 90.00, 128.74, 90.00 |
Refinement procedure
Resolution | 29.070 - 2.240 |
R-factor | 0.181 |
Rwork | 0.181 |
R-free | 0.22200 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1SSS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.330 * |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.900 | 2.390 |
High resolution limit [Å] | 2.240 | 2.240 |
Rmerge | 0.093 * | 0.255 * |
Number of reflections | 19994 | |
<I/σ(I)> | 13.6 | 5.2 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 * | 21 * | CRYSTALS WERE GROWN BY VAPOR DIFFUSION IN HANGING DROPS AT 21C IN A 1 TO 1 MIX OF THE RESERVOIR SOLUTION (8% PEG 8000, 0.1 M TRIS/HCL PH 8.5) AND A PROTEIN SOLUTION (1.45 MG/ML Y41F-SSSOD, 20 MM TRIS/HCL PH 7.8, 1% GLYCEROL). |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 8 (%) | |
2 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
3 | 1 | drop | protein | 1.45 (mg/ml) | |
4 | 1 | drop | Tris-HCl | 20 (mM) | pH7.8 |
5 | 1 | drop | glycerol | 1 (%) |