1WAB
PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Collection date | 1995-11-25 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 81.260, 81.260, 72.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.500 - 1.700 |
Rwork | 0.195 |
RMSD bond length | 0.015 |
RMSD bond angle | 0.034 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | PROLSQ |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 * |
High resolution limit [Å] | 1.650 * |
Rmerge | 0.063 |
Total number of observations | 387544 * |
Number of reflections | 33716 |
Completeness [%] | 99.7 |
Redundancy | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2.15-3.15 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 5 (mM) | |
3 | 1 | drop | DTT | 1 (mM) | |
4 | 1 | drop | ammonium sulfate | 8 (%sat) | |
5 | 1 | drop | sodium acetate | 100 (mM) | |
6 | 1 | reservoir | ammonium sulfate | 16 (%sat) | |
7 | 1 | reservoir | sodium acetate | 200 (mM) |