1VJS
STRUCTURE OF ALPHA-AMYLASE PRECURSOR
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-06-30 |
| Detector | FUJI |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 119.900, 119.900, 85.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.700 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.22600 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.720 |
| Data reduction software | WEIS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.700 * |
| Rmerge | 0.074 |
| Total number of observations | 504225 * |
| Number of reflections | 60922 |
| Completeness [%] | 88.6 |
| Redundancy | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | Lee, S.Y., (1991) Arch.Biochem.Biophys., 291, 255. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 1.0 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.0 (M) | |
| 3 | 1 | reservoir | ADA | 0.10 (M) |
