1VHQ
Crystal structure of enhancing lycopene biosynthesis protein 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Detector technology | CCD |
Detector | MARRESEARCH |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.090, 45.208, 76.332 |
Unit cell angles | 90.00, 118.78, 90.00 |
Refinement procedure
Resolution | 37.140 - 1.650 |
Rwork | 0.220 |
R-free | 0.26300 |
Structure solution method | Se-Met SAD phasing |
RMSD bond length | 0.014 |
RMSD bond angle | 2.300 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (4.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.140 | 1.740 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.078 | 0.812 |
Number of reflections | 53390 | |
<I/σ(I)> | 12.2 | 1.9 |
Completeness [%] | 97.5 | 87.9 |
Redundancy | 7.1 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
2 | 1 | drop | 150 (mM) | ||
3 | 1 | drop | methionine | 10 (mM) | |
4 | 1 | drop | glycerol | 10 (%) | |
5 | 1 | drop | dithiothreitol | 5 (mM) | |
6 | 1 | drop | protein | 10 (mg/ml) |