1VF4
cGSTA1-1 apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 123 |
Detector technology | IMAGE PLATE |
Collection date | 2000-10-10 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 55.115, 84.832, 114.256 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.900 - 2.450 |
Rwork | 0.206 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vf1 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.900 | 2.560 |
High resolution limit [Å] | 2.440 | 2.450 |
Rmerge | 0.064 | 0.410 |
Number of reflections | 9881 | |
<I/σ(I)> | 10 | 3.08 |
Completeness [%] | 95.8 | 96.1 |
Redundancy | 4.44 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | sodium cacodylate, magnesium acetate, PEG8000, ethacrynic acid, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |