1VF1
cGSTA1-1 in complex with glutathione
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 123 |
Detector technology | IMAGE PLATE |
Collection date | 1999-03-26 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 55.159, 85.609, 114.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.360 - 1.770 |
Rwork | 0.192 |
R-free | 0.21700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.360 | 1.830 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.058 | 0.401 |
Number of reflections | 27088 | |
<I/σ(I)> | 8.9 | 2.03 |
Completeness [%] | 99.0 | 99.3 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | sodium cacodylate, magnesium acetate, PEG8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |