1VEM
Crystal Structure Analysis of Bacillus Cereus Beta-Amylase at the optimum pH (6.5)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 2002-09-02 |
Detector | SIEMENS HI-STAR |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.576, 92.752, 65.787 |
Unit cell angles | 90.00, 102.36, 90.00 |
Refinement procedure
Resolution | 14.930 - 1.850 |
R-factor | 0.176 |
Rwork | 0.176 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b9z |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.900 | 1.780 |
High resolution limit [Å] | 1.700 | 1.720 |
Number of reflections | 63534 | |
Completeness [%] | 88.9 | 44.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | PEG 6000, ammonium sulfate, potassium phosphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |