1VE2
Crystal structure of uroporphyrin-III-C-methyltransferase from thermus thermophilus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-14 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.0, 0.979157, 0.979498 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.609, 87.924, 91.602 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.980 - 1.800 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.24100 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.055 | 0.336 |
| Number of reflections | 39564 | |
| Completeness [%] | 98.7 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | PEG4000, 0.1M HEPES-NaOH, 0.5M CALCIUM CHLORIDE, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
