1VAS
ATOMIC MODEL OF A PYRIMIDINE DIMER SPECIFIC EXCISION REPAIR ENZYME COMPLEXED WITH A DNA SUBSTRATE: STRUCTURAL BASIS FOR DAMAGED DNA RECOGNITION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1994-06-03 |
Detector | MACSCIENCE DIP100 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 65 |
Unit cell lengths | 118.820, 118.820, 36.280 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.750 |
R-factor | 0.152 |
Rwork | 0.152 |
R-free | 0.24000 |
RMSD bond length | 0.015 * |
RMSD bond angle | 1.980 * |
Data reduction software | ELMS |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.750 |
Rmerge | 0.062 |
Total number of observations | 24907 * |
Number of reflections | 5662 |
Completeness [%] | 72.0 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 4 * | pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 277.00K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | BIS-TRIS-PROPANE_HCL | ||
3 | 1 | 1 | NH4 SULFATE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.38 (mM) | |
2 | 1 | drop | DNA | 0.53 (mM) | duplex |
3 | 1 | drop | Tris-HCl | 150 (mM) | |
4 | 1 | drop | ammonium sulfate | 33 (%sat) | |
5 | 1 | reservoir | ammonium sulfate | 54 (%sat) |