1V5C
The crystal structure of the inactive form chitosanase from Bacillus sp. K17 at pH3.7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-10 |
Detector | OXFORD PX210 |
Wavelength(s) | 0.90 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 85.063, 91.145, 131.524 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.510 - 2.000 |
Rwork | 0.184 |
R-free | 0.20800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.510 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.072 | 0.275 |
Number of reflections | 34863 | |
<I/σ(I)> | 9.4 | 3 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.19 | 7.39 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.7 | 277 | PEG 4000, ammonium sulfate, sodium citrate, pH 3.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |