1V3R
Crystal structure of TT1020 from Thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-12-12 |
Detector | MARRESEARCH |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.520, 75.185, 76.051 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.560 - 1.850 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.22500 |
Structure solution method | MIR |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.055 | 0.283 |
Number of reflections | 26776 | |
Completeness [%] | 99.8 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | NaCl, sodium acetate, Tris, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |