1UW3
The crystal structure of the globular domain of sheep prion protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 165 |
Detector technology | CCD |
Collection date | 2003-06-15 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 85.068, 29.045, 45.896 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.050 |
R-factor | 0.227 |
Rwork | 0.222 |
R-free | 0.31900 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i4m |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 2.130 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.103 * | 0.465 * |
Number of reflections | 7095 | |
<I/σ(I)> | 6 | 2 |
Completeness [%] | 93.2 | 83.7 * |
Redundancy | 2.5 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.6 | pH 8.60 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7 (mg/ml) | |
2 | 1 | reservoir | sodium potassium phosphate | 0.8-1.3 (M) | pH7.4-8.6 |