1ULX

Partially photolyzed structure of CO-bound heme-heme oxygenase complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	35
Detector technology	CCD
Collection date	2003-06-02
Detector	MARRESEARCH
Wavelength(s)	1.000
Spacegroup name	P 32 2 1
Unit cell lengths	65.900, 65.900, 120.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.209
Rwork	0.207
R-free	0.23900
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1ix4
RMSD bond length	1.204
RMSD bond angle	0.007
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	17805
<I/σ(I)>	18.6
Completeness [%]	84.8	87
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	Sodium formate, Potassium phosphate, Sodium azide, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K