1UJ0
Crystal Structure of STAM2 SH3 domain in complex with a UBPY-derived peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-10-02 |
Detector | RIGAKU |
Wavelength(s) | 1.00 |
Spacegroup name | P 61 |
Unit cell lengths | 48.454, 48.454, 58.582 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.110 - 1.700 |
R-factor | 0.2163 |
Rwork | 0.215 |
R-free | 0.23000 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.021 |
RMSD bond angle | 1.673 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | EPMR |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.100 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.064 * | |
Number of reflections | 8501 | |
Completeness [%] | 97.9 | 96.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 25 * | 0.08M Sodium Phosphate monobasic, 1.92M Potassium phosphate dibasic, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4.6 (mg/ml) | |
2 | 1 | reservoir | sodium phosphate | 0.08 (M) | |
3 | 1 | reservoir | potassium phosphate | 1.92 (M) |