1UFY
Crystal analysis of chorismate mutase from thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-11-07 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 0.8 |
Spacegroup name | H 3 2 |
Unit cell lengths | 71.252, 71.252, 152.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 0.960 |
R-factor | 0.1099 |
Rwork | 0.110 |
R-free | 0.12740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PROTOMER A OF PDB ENTRY 1ODE |
RMSD bond length | 0.018 |
RMSD bond angle | 0.031 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 0.960 |
High resolution limit [Å] | 0.950 | 0.950 |
Rmerge | 0.050 | 0.345 |
Number of reflections | 90841 | |
<I/σ(I)> | 22.2 | 1.5 |
Completeness [%] | 97.2 | 59.7 |
Redundancy | 4.7 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7 | 291 | SODIUM MALONATE, MES, pH 7.0, MICRO BATCH, temperature 291K |