1UC3
Crystal Structure of hemoglobin I from river lamprey
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 100 |
Detector technology | DIFFRACTOMETER |
Collection date | 1999-06-08 |
Detector | WEISSENBERG |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 59.210, 73.610, 105.870 |
Unit cell angles | 103.95, 91.52, 97.64 |
Refinement procedure
Resolution | 10.000 - 2.300 |
Rwork | 0.197 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lhb |
RMSD bond length | 0.010 |
RMSD bond angle | 1.141 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.350 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.072 | 0.255 |
Number of reflections | 76404 | |
<I/σ(I)> | 14.6 | 3.4 |
Completeness [%] | 97.8 | 94.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 283 | 0.1M HEPES, 21% PEG6000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 283K |