1UAA
E. COLI REP HELICASE/DNA COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-04-07 |
Detector | MARRESEARCH |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 141.800, 141.800, 284.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 3.000 |
R-factor | 0.228 * |
Rwork | 0.228 |
R-free | 0.32800 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.918 * |
Data reduction software | DENZO |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 3.000 |
Rmerge | 0.053 |
Total number of observations | 434546 * |
Number of reflections | 57472 |
Completeness [%] | 97.3 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 | 20 * | pH 8.00, VAPOR DIFFUSION, temperature 293.00K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | NACL | ||
3 | 1 | 1 | TRIS-HCL | ||
4 | 1 | 1 | EDTA | ||
5 | 1 | 1 | GLYCEROL | ||
6 | 1 | 2 | WATER | ||
7 | 1 | 2 | GLYCEROL |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | glycerol | 10 (%(v/v)) | |
2 | 1 | drop | 0.1 (M) | ||
3 | 1 | drop | Tris-HCl | 50 (mM) | |
4 | 1 | drop | 1 (mM) | ||
5 | 1 | drop | protain | 20 (mg/ml) | |
6 | 1 | drop | PEG8000 | 2.5 (%) | |
7 | 1 | reservoir | glycerol | 2 (%(v/v)) |