1TYG
Structure of the thiazole synthase/ThiS complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 8-BM |
Synchrotron site | APS |
Beamline | 8-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 91.654, 91.654, 401.300 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 3.150 |
R-factor | 0.24691 |
Rwork | 0.242 |
R-free | 0.30460 |
Structure solution method | SAD |
RMSD bond length | 0.022 |
RMSD bond angle | 2.022 |
Data reduction software | MOSFLM |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 3.260 |
High resolution limit [Å] | 3.150 | 3.150 |
Number of reflections | 17866 | |
Completeness [%] | 98.2 | 99.8 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.15 | 293 | 5% PEG 8K, 200 mM Sodium Chloride, 100 mM sodium phosphate pH 6.1, pH 7.15, VAPOR DIFFUSION, HANGING DROP, temperature 293K |