1TVR
HIV-1 RT/9-CL TIBO
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Detector technology | CCD |
Collection date | 1994-11 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 226.000, 69.300, 104.100 |
Unit cell angles | 90.00, 107.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 3.000 |
R-factor | 0.259 |
Rwork | 0.259 |
RMSD bond length | 0.015 |
RMSD bond angle | 2.200 |
Data reduction software | DENZO |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 * |
High resolution limit [Å] | 3.000 * |
Rmerge | 0.064 |
Total number of observations | 56695 * |
Number of reflections | 26209 |
Completeness [%] | 84.0 |
Redundancy | 2.16 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.8 * | 4 * | Clark, A.D., (1995) Methods Enzymol., 262, 171. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 19-20 (mg/ml) | |
2 | 1 | drop | beta-OG | 0.1 (%) | |
3 | 1 | reservoir | Bis-Tris propane | 50 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 100 (mM) | |
5 | 1 | reservoir | glycerol | 10 (%) | |
6 | 1 | reservoir | PEG8000 | 12 (%(w/v)) | |
7 | 1 | drop | DMSO | 1.6 (%) |