1TRE
THE STRUCTURE OF TRIOSEPHOSPHATE ISOMERASE FROM ESCHERICHIA COLI DETERMINED AT 2.6 ANGSTROM RESOLUTION
Experimental procedure
Detector technology | IMAGE PLATE |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.690, 46.780, 151.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 * - 2.600 |
R-factor | 0.119 |
Rwork | 0.119 |
RMSD bond length | 0.018 |
RMSD bond angle | 2.400 |
Phasing software | X-PLOR |
Refinement software | TNT |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.600 * |
Rmerge | 0.083 * |
Total number of observations | 51476 * |
Number of reflections | 12801 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.6 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
10 | 1 | reservoir | dithiothreitol | 1 (mM) | |
11 | 1 | reservoir | EDTA | 1 (mM) | |
12 | 1 | reservoir | 1 (mM) | ||
2 | 1 | drop | Tris/Hcl | 25 (mM) | |
3 | 1 | drop | 20 (mM) | ||
4 | 1 | drop | EDTA | 1 (mM) | |
5 | 1 | drop | dithiothreitol | 1 (mM) | |
6 | 1 | drop | 1 (mM) | ||
7 | 1 | drop | 2-phosphoglycolate | 1 (mM) | |
8 | 1 | reservoir | PEG6000 | 22 (%) | |
9 | 1 | reservoir | MES | 200 (mM) |