1TO5
Structure of the cytosolic Cu,Zn SOD from S. mansoni
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-01-19 |
Detector | RIGAKU RAXIS II |
Wavelength(s) | 1.5408 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.640, 78.240, 95.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.800 - 2.200 |
Rwork | 0.176 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.270 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 25229 | |
<I/σ(I)> | 17.3 | 6.3 |
Completeness [%] | 86.5 | 72.9 |
Redundancy | 4.5 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 30% PEG 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |