1TLG
STRUCTURE OF A TUNICATE C-TYPE LECTIN COMPLEXED WITH D-GALACTOSE
Experimental procedure
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-13 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-08 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.180, 66.450, 85.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.000 - 2.200 |
R-factor | 0.211 * |
Rwork | 0.211 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1byf |
RMSD bond length | 0.007 |
RMSD bond angle | 0.025 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 16.400 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.056 | 0.171 |
Total number of observations | 108587 * | |
Number of reflections | 16111 | |
<I/σ(I)> | 11.6 | 4.4 |
Completeness [%] | 99.1 | 83.8 |
Redundancy | 6.7 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.2 * | 17 * | pH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.4 (mM) | |
2 | 1 | drop | 12.5 (mM) | ||
3 | 1 | drop | D-galactose | 2.5 (mM) | |
4 | 1 | drop | Tris | 10 (mM) | |
5 | 1 | drop | 25 (mM) | ||
6 | 1 | reservoir | PEG8000 | 7 (%(w/v)) | |
7 | 1 | reservoir | 0.2 (M) | ||
8 | 1 | reservoir | sodium cacodylate | 0.1 (M) |