1TFH
EXTRACELLULAR DOMAIN OF HUMAN TISSUE FACTOR
Experimental procedure
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-18 |
Temperature [K] | 297 |
Detector technology | AREA DETECTOR |
Collection date | 1994-12 |
Detector | SIEMENS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.393, 85.828, 112.901 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.400 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.29700 |
Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT + MOLECULAR REPLACEMENT |
Starting model (for MR) | C ALPHA COORDINATES OF PDB ENTRY 1BOY |
RMSD bond length | 0.013 |
RMSD bond angle | 27.100 * |
Data reduction software | XENGEN |
Data scaling software | XENGEN |
Phasing software | AMoRE |
Refinement software | XTALVIEW |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.000 | 2.420 |
High resolution limit [Å] | 2.270 | 2.270 |
Rmerge | 0.073 * | 0.436 * |
Total number of observations | 64590 * | |
Number of reflections | 26432 | |
<I/σ(I)> | 21 | 1.5 |
Completeness [%] | 90.0 | 52.6 |
Redundancy | 2.4 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6.5 * | 278 | CRYSTAL CAN BE GROWN FROM 20MM PHOSPHATE BUFFER, PH 6.5, AT A 40MG/ML PROTEIN CONCENTRATION. THE CRYSTALS USED FOR DIFFRACTION STUDIES WERE IN FACT CRYSTALLIZED UNEXPECTEDLY IN 2 DAYS IN AN NMR TUBE CONTAINING A SOLUTION OF APPROXIMATELY 3.5MM OF UNIFORMLY 15N-ENRICHED (~99 ATOM%) TISSUE FACTOR (AT ~85 MG/ML) IN 20MM KNA2PO4, 0.02%NAN3, 10% D2O AND 90% H2O, PH 7.0 AT 5 DEGREE., temperature 278K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | phosphate | 20 (mM) | |
2 | 1 | 1 | protein | 40 (mg/ml) |