1TF9
Streptomyces griseus aminopeptidase complexed with P-Iodo-L-Phenylalanine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2001-09-27 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 61.053, 61.053, 144.502 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.300 |
Rwork | 0.122 |
R-free | 0.17220 |
Structure solution method | Difference fourier from previously determined, related structure |
RMSD bond length | 0.012 |
RMSD bond angle | 0.028 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.320 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 63168 | |
<I/σ(I)> | 17.2 | |
Completeness [%] | 92.7 | 57.8 |
Redundancy | 11 | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | PEG 4000, sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |