1T3G
Crystal structure of the Toll/interleukin-1 receptor (TIR) domain of human IL-1RAPL
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9793, 0.9792, 0.9200 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.987, 53.265, 183.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.880 - 2.300 |
R-factor | 0.228 |
Rwork | 0.228 |
R-free | 0.27200 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.300 |
Rmerge | 0.054 |
Number of reflections | 41323 |
Completeness [%] | 94.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | PEG 4000, SODIUM ACETATE, DTT, GLYCEROL, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |