1T1D
CRYSTAL STRUCTURE OF THE TETRAMERIZATION DOMAIN OF THE SHAKER POTASSIUM CHANNEL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-15 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 59.710, 59.710, 146.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.800 - 1.510 |
| R-factor | 0.229 * |
| Rwork | 0.229 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a68 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.026 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.540 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.066 * | |
| Total number of observations | 135247 * | |
| Number of reflections | 24631 * | |
| <I/σ(I)> | 10.2 | 3.5 |
| Completeness [%] | 93.7 * | 93.7 |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 24% ISOPROPANOL, .2M MGCL2, .1 M HEPES PH 7.5, 1MM DTT |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium formate | 2.5 (M) | |
| 2 | 1 | reservoir | bis-Tris-propane | 0.1 (M) | |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | 0.15 (M) | ||
| 5 | 1 | reservoir | beta-mercaptoethanol | 10 (mM) | |
| 6 | 1 | drop | isopropanol | 24 (%) | |
| 7 | 1 | drop | 0.2 (M) | ||
| 8 | 1 | drop | HEPES | 0.1 (M) | pH7.5 |
| 9 | 1 | drop | dithiothreitol | 1 (mM) |
