1SZW
Crystal structure of E. coli tRNA pseudouridine synthase TruD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.93219 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.180, 108.110, 112.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.000 |
| R-factor | 0.19397 |
| Rwork | 0.192 |
| R-free | 0.23913 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.659 |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.060 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 52753 | |
| <I/σ(I)> | 15.9 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 5000MME, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
