1SZB
Crystal structure of the human MBL-associated protein 19 (MAp19)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-21 |
Detector | MARRESEARCH |
Wavelength(s) | 0.946 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 67.754, 67.754, 187.923 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 17.000 - 2.500 |
R-factor | 0.263 |
Rwork | 0.261 |
R-free | 0.31600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nt0 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 15712 | |
<I/σ(I)> | 23.7 | 5.1 |
Completeness [%] | 94.8 | 86.1 |
Redundancy | 3.5 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG8000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |