1SLF
APOSTREPTAVIDIN, PH 5.6, TWO MOLECULES OF (SO4)2 BOUND AT THE BIOTIN BINDING SITE
Experimental procedure
Detector technology | AREA DETECTOR |
Detector | SIEMENS |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 95.430, 106.120, 47.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.500 - 1.740 * |
R-factor | 0.176 |
Rwork | 0.176 |
R-free | 0.20200 |
RMSD bond length | 0.018 |
RMSD bond angle | 27.800 * |
Data reduction software | SADIE |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 * |
High resolution limit [Å] | 1.740 * |
Rmerge | 0.073 |
Total number of observations | 93159 * |
Number of reflections | 22185 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.6 | pH 5.6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 30-32 (mg/ml) | |
2 | 1 | reservoir | potassium acetate | 0.1 (M) | |
3 | 1 | drop | protein | 30 (mg/ml) |