1SHT
Crystal Structure of the von Willebrand factor A domain of human capillary morphogenesis protein 2: an anthrax toxin receptor
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-12 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9186, 1.0723, 1.0332, 1.0720 |
Spacegroup name | P 21 3 |
Unit cell lengths | 79.691, 79.691, 79.691 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.810 |
R-factor | 0.15149 |
Rwork | 0.150 |
R-free | 0.18636 |
Structure solution method | MAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.626 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.065 | 0.426 |
Number of reflections | 15750 | |
<I/σ(I)> | 34.3 | 4.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG8K, magnesium acetate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |