1SGM
Crystal Structure of Hypothetical Protein YXAF
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9788, 0.9792, 0.930 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.356, 38.956, 95.705 |
| Unit cell angles | 90.00, 101.26, 90.00 |
Refinement procedure
| Resolution | 33.010 - 2.000 |
| R-factor | 0.249 |
| Rwork | 0.233 |
| R-free | 0.29400 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | |
| Number of reflections | 31966 | |
| Completeness [%] | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | BiS-Tris, MPD, PEG 1500, Ammonium Acetate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
