1SGH
Moesin FERM domain bound to EBP50 C-terminal peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 123 |
Detector technology | CCD |
Collection date | 2000-05-04 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.943 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 127.600, 70.500, 62.800 |
Unit cell angles | 90.00, 106.10, 90.00 |
Refinement procedure
Resolution | 11.070 - 3.500 |
Rwork | 0.338 |
R-free | 0.40100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ef1 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.750 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 11.070 | 3.690 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.101 | 0.471 |
Number of reflections | 6801 | |
<I/σ(I)> | 6.6 | 2 |
Completeness [%] | 99.2 | 99.2 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | Tris, PEG 4000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |