1S8O
Human soluble Epoxide Hydrolase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-01-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 92.546, 92.546, 244.637 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.410 - 2.600 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cqz |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.100 | 0.363 |
Number of reflections | 19879 | |
<I/σ(I)> | 30.9 | 11.5 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 9.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.4 | 277 | PEG 4000, Tris, lithium sulfate, pH 8.4, VAPOR DIFFUSION, temperature 277K |