1S7I
1.8 A Crystal Structure of a Protein of Unknown Function PA1349 from Pseudomonas aeruginosa
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-11 |
Detector | SBC-2 |
Wavelength(s) | 0.9795, 0.9797, 0.94656 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 103.437, 103.437, 103.437 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.690 - 1.800 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.26200 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.048 | 0.680 |
Number of reflections | 17179 | |
<I/σ(I)> | 41.33 | 1.71 |
Completeness [%] | 94.8 | 76.8 |
Redundancy | 9.12 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 1.5M Amm. Sulph., 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |