1RYW
C115S MurA liganded with reaction products
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 85 |
Detector technology | IMAGE PLATE |
Collection date | 2000-06-14 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.867, 153.729, 135.089 |
Unit cell angles | 90.00, 104.17, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.178 |
Rwork | 0.178 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ejc |
RMSD bond length | 0.007 |
RMSD bond angle | 1.320 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 140111 | |
<I/σ(I)> | 12 | 3 |
Completeness [%] | 98.9 | 97.3 |
Redundancy | 6 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | PEG 20,000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |