1RYL
The Crystal Structure of a Protein of Unknown Function YfbM from Escherichia coli
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-12 |
Detector | SBC-2 |
Wavelength(s) | 0.9795,0.9797,0.94656 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 103.848, 60.455, 57.931 |
Unit cell angles | 90.00, 114.62, 90.00 |
Refinement procedure
Resolution | 30.230 - 1.600 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.25900 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.100 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.055 | 0.372 |
Number of reflections | 78702 | |
<I/σ(I)> | 15.85 | 1.223 |
Completeness [%] | 93.2 | 55.5 |
Redundancy | 2.37 | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.2 M MgCl2, 0.1 M HEPES, 30%PEG3350, 8% Ethanol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |