1RWB
Cooperative Effect of Two Surface Amino Acid Mutations (Q252L and E170K) of Glucose Dehydrogenase from Bacillus megaterium IWG3 for the stabilization of Oligomeric State
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-10-05 |
Detector | MARRESEARCH |
Wavelength(s) | 0.95 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 118.500, 65.400, 118.000 |
Unit cell angles | 90.00, 92.60, 90.00 |
Refinement procedure
Resolution | 15.000 * - 2.000 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.26700 |
Structure solution method | Rigid Body |
Starting model (for MR) | 1gco |
RMSD bond length | 0.006 |
RMSD bond angle | 22.300 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 * | 1.920 |
High resolution limit [Å] | 2.000 * | 1.830 |
Rmerge | 0.057 * | 0.119 * |
Total number of observations | 193166 * | |
Number of reflections | 61998 * | |
<I/σ(I)> | 7 | 3.6 |
Completeness [%] | 99.4 | 99.4 |
Redundancy | 3.1 * | 3.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 * | 291 | PEG 6000, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 10 (mg/ml) | |
2 | 1 | reservoir | NAD+ | 4 (mM) | |
3 | 1 | reservoir | sodium phosphate | 50 (mM) | pH6.3 |