1RVW
R STATE HUMAN HEMOGLOBIN [ALPHA V96W], CARBONMONOXY
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-02 |
| Detector | SIEMENS |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 54.282, 54.282, 194.122 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.500 |
| R-factor | 0.167 |
| Rwork | 0.167 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 1hho |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.610 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.065 | |
| Number of reflections | 9340 | 993 * |
| Completeness [%] | 86.7 | 75.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | Perutz, M.F., (1968) J.Crystal Growth, 2, 54. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 4 (M) | ||
| 2 | 1 | 1 | 2 (M) | ||
| 3 | 1 | 1 | 2 (M) |
