1RP8
Crystal structure of barley alpha-amylase isozyme 1 (amy1) inactive mutant d180a in complex with maltoheptaose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-12-13 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9340 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 93.000, 72.500, 62.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.140 - 2.000 |
R-factor | 0.172 |
Rwork | 0.172 |
R-free | 0.22100 |
Structure solution method | FOURIER DIFFERENCE |
Starting model (for MR) | 1ht6 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.400 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.140 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 29146 | |
<I/σ(I)> | 7.5 | 2.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.3 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 290 | PEG 8000, ISOPROPANOL, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 290K |