1RGS
REGULATORY SUBUNIT OF CAMP DEPENDENT PROTEIN KINASE
Experimental procedure
Detector technology | AREA DETECTOR |
Collection date | 1993 |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 88.900, 88.900, 179.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 2.800 |
R-factor | 0.217 |
Rwork | 0.217 |
RMSD bond length | 0.019 |
RMSD bond angle | 2.800 |
Data reduction software | UCSD |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.800 * |
Rmerge | 0.055 * |
Number of reflections | 16785 * |
Completeness [%] | 94.0 * |
Redundancy | 3.6 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.5 * | 22 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 0.55-0.6 (M) | |
3 | 1 | drop | glycerol | 5-6.25 (%) | |
4 | 1 | drop | dithiothreitol | 5 (mM) | |
5 | 1 | drop | sodium acetate | 40-50 (mM) | |
6 | 1 | reservoir | ammonium sulfate | 1.1-1.2 (M) | |
7 | 1 | reservoir | glycerol | 10-12.5 (%) | |
8 | 1 | reservoir | dithiothreitol | 10 (mM) | |
9 | 1 | reservoir | sodium acetate | 80-100 (mM) |