1RGN
Structure of the reaction centre from Rhodobacter sphaeroides carotenoidless strain R-26.1 reconstituted with spheroidene
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-05-14 |
Detector | MARRESEARCH |
Wavelength(s) | 0.933 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 139.817, 139.817, 184.047 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.000 - 2.800 |
R-factor | 0.19076 |
Rwork | 0.189 |
R-free | 0.23246 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.907 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.055 | 0.342 |
Number of reflections | 46063 | |
<I/σ(I)> | 22.5 | 3.2 |
Completeness [%] | 89.0 | 91.4 |
Redundancy | 3.9 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | Trisodium citrate, LDAO, 1,2,3-heptanetriol, ethylene glycol, Tris-HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 16.0K |