1REW
Structural refinement of the complex of bone morphogenetic protein 2 and its type IA receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-08-16 |
Detector | MARRESEARCH |
Wavelength(s) | 0.918 |
Spacegroup name | P 65 |
Unit cell lengths | 107.493, 107.493, 102.282 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.730 - 1.863 |
R-factor | 0.2085 |
Rwork | 0.207 |
R-free | 0.22943 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1es7 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.442 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | REFMAC (5.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.730 | 1.910 |
High resolution limit [Å] | 1.860 | 1.860 |
Number of reflections | 54306 | |
Completeness [%] | 96.8 | 94.9 |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 280 | sodium acetate, imidazole, glucose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 280K |