1RED
ENDO-1,4-BETA-XYLANASE II COMPLEX WITH 4,5-EPOXYPENTYL-BETA-D-XYLOSIDE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1995-07-10 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.940, 60.960, 38.040 |
| Unit cell angles | 90.00, 94.03, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.600 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.22200 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 * |
| Data reduction software | R-AXIS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 1.600 * | 1.600 * |
| Rmerge | 0.073 | |
| Total number of observations | 88868 * | |
| Number of reflections | 38005 | |
| Completeness [%] | 76.0 | 25.9 * |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.2 * | Torronen, A., (1993) J. Mol. Biol., 233, 313. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 50 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 25 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 25 (%) |
