1R5T
The Crystal Structure of Cytidine Deaminase CDD1, an Orphan C to U editase from Yeast
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-09-17 |
Detector | APS-1 |
Wavelength(s) | 1.2574, 1.2831, 1.2828 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 78.520, 86.370, 156.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.050 - 2.000 |
R-factor | 0.188 |
Rwork | 0.188 |
R-free | 0.22200 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 63560 | |
<I/σ(I)> | 17.7 | 2.1 |
Completeness [%] | 91.5 | 74.8 |
Redundancy | 4.5 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | PEG 5000 MME, ammonium chloride, sodium succinate, DTT, sodium azide, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | PEG 5000 MME, ammonium chloride, sodium succinate, DTT, sodium azide, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | PEG 5000 MME, ammonium chloride, sodium succinate, DTT, sodium azide, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |