1QX1
Golgi alpha-mannosidase II D341N mutant complex with 2-F-mannosyl-F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.046, 109.826, 138.907 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 1.300 |
| R-factor | 0.17 |
| Rwork | 0.170 |
| R-free | 0.18800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hty |
| RMSD bond length | 0.020 * |
| RMSD bond angle | 2.020 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.380 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.106 | 0.586 |
| Total number of observations | 1589410 * | |
| Number of reflections | 246051 | |
| <I/σ(I)> | 14.7 | 2.9 |
| Completeness [%] | 95.3 | 89.7 * |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 298 | van den Elsen, J.M., (2001) EMBO J., 20, 3008. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 8.5 (%) | |
| 2 | 1 | reservoir | MPD | 5 (%) | |
| 3 | 1 | reservoir | Tris | 100 (mM) | pH7.0 |
