1QS2
CRYSTAL STRUCTURE OF VIP2 WITH NAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-03-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.673, 70.564, 142.612 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.26600 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 21.260 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.098 | 0.309 |
| Total number of observations | 118889 * | |
| Number of reflections | 15370 | |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 97.9 | 99.9 |
| Redundancy | 7.7 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | 20 * | PEG200, 2-METHYL 2,4-PENTANEDIOL, NAD, BIS-TRIS, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | |
| 3 | 1 | reservoir | PEG200 | 30 (%(w/v)) | |
| 4 | 1 | reservoir | octyl-beta-D-glucopyranoside | 1 (%(w/v)) | |
| 5 | 1 | reservoir | imidazole/malate | 200 (mM) |
