1QRT

GLUTAMINYL-TRNA SYNTHETASE MUTANT D235G COMPLEXED WITH GLUTAMINE TRANSFER RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Detector technology	AREA DETECTOR
Collection date	1990-09-28
Detector	SDMS
Spacegroup name	C 2 2 21
Unit cell lengths	243.500, 93.700, 115.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.500 - 2.700
R-factor	0.216
Rwork	0.216
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	SDMS
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	2.700 *
Rmerge	0.062
Number of reflections	33823
Completeness [%]	95.7
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		17 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-15 (mg/ml)
2	1	reservoir	PIPES	80 (mM)
3	1	reservoir		20 (mM)
4	1	reservoir		0.02 (%)
5	1	reservoir	ammonium sulfate	1.8-2.0 (M)
6	1	reservoir	ATP	8 (mM)