1QOM
MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DIMER (DELTA 65) WITH SWAPPED N-TERMINAL HOOK
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-09-15 |
Detector | MARRESEARCH |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 212.980, 212.980, 114.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
R-factor | 0.236 |
Rwork | 0.236 |
R-free | 0.30600 |
Structure solution method | OTHER |
RMSD bond length | 0.008 |
RMSD bond angle | 23.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.8) |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.059 * | 0.362 * |
Total number of observations | 153562 * | |
Number of reflections | 39200 | 4290 * |
<I/σ(I)> | 17 | 2.9 |
Completeness [%] | 91.7 | 92.3 |
Redundancy | 3.9 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.6 * | 4 * | pH 6.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 17 (mg/ml) | |
2 | 1 | drop | Hepps | 40 (mM) | |
3 | 1 | drop | glycerol | 10 (%) | |
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | drop | H4B | 2-4 (mM) | |
6 | 1 | reservoir | beta-octyl-glucoside | 50 (mM) | |
7 | 1 | reservoir | 18-21 (%) | or ammonium sulfate | |
8 | 1 | reservoir | S-ethyl-thiourea | 4 (mM) | or 4mM Fe(II)Cl2 |