1QMO
Structure of FRIL, a legume lectin that delays hematopoietic progenitor maturation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 151.360, 151.360, 309.880 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 3.500 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | MODEL |
RMSD bond length | 0.008 |
RMSD bond angle | 26.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (0.4) |
Refinement software | CNS (0.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.620 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.159 * | |
Total number of observations | 257389 * | |
Number of reflections | 26919 * | |
<I/σ(I)> | 11.3 | 4.8 |
Completeness [%] | 99.4 | |
Redundancy | 12.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | METHOD 'HANING DROP BOTTOM', 20 % PEG 8000, 0.1 M NACACODYLATE, PH 6.5, 0.2 M (NH4)2SO4, WITH A 5 MICROLITER PROTEIN SOLUTION DROP, (4.3 MG/ML)+ 5 MICROLITER BOTTOM SOLUTION + 1 MICROLITER TRIMANNOSIDE SOLUTION (90 MM) |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4.3 (mg/ml) | |
2 | 1 | drop | Man(alpha1-3)[Man(alpha1-6)]Man(alpha1-O-Me) | 90 (mM) | |
3 | 1 | drop | bottom solution | ||
4 | 1 | reservoir | PEG8000 | 11 (%) | bottom solution |
5 | 1 | reservoir | Na cacodylate | 0.1 (M) | bottom solution |
6 | 1 | reservoir | ammonium sulfate | 0.2 (M) | bottom solution |