1QHF
YEAST PHOSPHOGLYCERATE MUTASE-3PG COMPLEX STRUCTURE TO 1.7 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.5 |
Synchrotron site | SRS |
Beamline | PX9.5 |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 96.400, 85.900, 81.900 |
Unit cell angles | 90.00, 120.60, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.700 |
Rwork | 0.177 |
R-free | 0.21500 |
Structure solution method | OTHER |
Starting model (for MR) | 3pgm |
RMSD bond length | 0.012 |
RMSD bond angle | 0.029 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.000 | 2.300 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.088 * | |
Number of reflections | 42944 | |
Completeness [%] | 82.5 | 77.2 |
Redundancy | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.8 | 277 * | 55% AMMONIUM SULFATE IN 10MM IMIDAZOLE BUFFER PH 6.8 WITH 1MM 3PG AND CONCENTRATION OF 10 MG ML PROTEIN |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 55 (%) | |
3 | 1 | reservoir | imidazole | 10 (mM) | |
4 | 1 | reservoir | 3PG substrate | 1 (mM) |